The queens must write and perform a stand-up comedy routine in front of a live audience. With guest judges Jaime Pressly (My Name Is Earl) and comedy writer Bruce Vilanch.

Cast: RuPaul, Santino Rice, Michelle Visage, Jaime Pressly, Bruce Vilanch, Shawn Morales, Simon Sherry-Wood, Roy Haylock, Jay Jackson, Shane Jenek, Joshua Jones, Patrick Joslyn, Greg Meyer, Danny Noriega, Benjamin Putnam